Extraction and Characterization of Flavanol-Rich Nutraceuticals Based on High-Performance Liquid Chromatography

Flavanols are a class of polyphenols that have become the focus much research because their medicinal properties. For this paper, new method to determine in nutraceutical samples was developed, based on solid-liquid extraction and further analysis by liquid chromatography. Analytes were recovered ultrasound-assisted extraction, using mixture methanol/water/hydrochloric acid (70:29:1, v:v:v) as solvent. Reversed-phase high-performance chromatography with ultraviolet/visible (HPLC-UV/Vis) fluorescence detection (HPLC-FLD) developed content principal flavanols. The HPLC-FLD showed better selectivity sensitivity, so it selected for studies. analytical parameters established FLD satisfactory terms linearity (R2 ≥ 0.993), repeatability (RSD% 2.2), limits 0.1 mg L−1 or below. analyzed compositional profiles flavanols, then they subsequently treated chemometric methods, such component (PCA). Results study from flavanols sufficient description discrimination purposes. cranberry richest procyanidin A2 being flavanol, artichoke least rich.